Figure 5From: Subgroup J avian leukosis virus infection inhibits autophagy in DF-1 cellsVerification of atg5+/+MEFs and atg5-/-MEFs. Following 48 h culture, atg5+/+ MEFs and atg5-/- MEFs were collected for western blot analysis using Atg5 rabbit monoclonal antibody. The Atg5 protein has an approximate M.W. of 55 kDa. The absence of this 55 kDa band confirmed Atg5 knockdown in atg5-/- MEFs. In contrast, this band was observed for atg5+/+ MEFs.Back to article page