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Figure 1 | Virology Journal

Figure 1

From: Subgroup J avian leukosis virus infection inhibits autophagy in DF-1 cells

Figure 1

ALV-J infection inhibits autophagy in DF-1 cells. (A) Fluorescence microscopy revealed limited numbers of GFP-LC3 puncta in ALV-J-infected cells. DF-1 cells were transfected with GFP-LC3, infected with mock or ALV-J strain HN06 at an MOI of 1 for 2 h, and cultured in DMEM (+10% FBS) for 48 h. Cells with punctuated GFP-LC3 were counted and displayed as a histogram. * P-value < 0.05. (B) DF-1 cells were infected with mock or ALV-J (MOI = 1), harvested at 6, 12, 24, 36, 48, 60, 72, 84 and 96 h p.i., the cells lysed and proteins analyzed by western blotting. (C) Autophagosomes were observed with transmission electron microscopy. Mock-infected cells showed autophagosomes with double-membranes and autophagic vacuoles. No autophagic vacuoles were observed for ALV-J infected cells (MOI = 1).

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