Figure 6
Non-specific inhibition of RSV in minimally diluted samples. Immunoglobulin-depleted human serum was assessed in qPCR-MN (Vero cells; 500 TCID50 per well of virus input; assessment at 24 hours post-infection) with (A) RSV-A2 and (B) RSV-B1. Values represent the mean ± SD (n = 9). Spiking experiments were also performed using the human immunoglobulin standard RSV-Lot 1. For the initial spiked sample, RSV-Lot 1 at 10% concentration was diluted 1:10 in immunoglobulin-depleted human serum. Five additional samples were generated by two-fold serial dilution using the depleted serum as the diluent. The resulting samples with simulated low neutralizing activity were assessed by qPCR-MN with (C) RSV-A2 and (D) RSV-B1. The observed GMT (n = 3) is plotted against the dilution factor of the simulated sample.