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Figure 3 | Virology Journal

Figure 3

From: A neutralization assay for respiratory syncytial virus using a quantitative PCR-based endpoint assessment

Figure 3

qPCR signal vs. input virus dose. Vero cells were seeded in 96-well culture plates (15,000 cells per well). On the following day, each well was infected with an inoculum from a two-fold dilution series: 40,000 to 78 TCID50 per well for (A) RSV-A2, and 250,000 to 490 TCID50 per well for (B) RSV-B1. At 24 hours post-infection, cell lysates were prepared using Bio-Rad SPR and subjected to qRT-PCR analysis. RNA copy numbers were normalized to the mean value obtained with the most dilute virus input. Each point represents the mean with corresponding range (n = 4).

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Virology Journal

ISSN: 1743-422X