Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

Table 2 SYBR Green PCR assay for TTV using single series of plasmid standards

Plasmid standards	Day	C_TMean ± SD (n=3)*	Intra-day precision	Inter-day precision	Intra-day accuracy	Inter-day accuracy
10 × 10⁹	1	4.21 ± 0.02	0.50	0.45	99.6	99.6
10 × 10⁹	2	4.18 ± 0.01	0.39		99.6
10 × 10⁸	1	7.59 ± 0.05	0.75	0.65	101.3	101.3
10 × 10⁸	2	7.52 ± 0.05	0.78		101.2
10 × 10⁷	1	11.74 ± 0.05	0.45	1.10	100.8	100.8
10 × 10⁷	2	11.56 ± 0.01	0.14		100.8
10 × 10⁶	1	15.83 ± 0.04	0.28	1.22	100.4	100.4
10 × 10⁶	2	15.56 ± 0.01	0.08		100.4
10 × 10⁵	1	19.75 ± 0.05	0.26	1.39	100.6	100.7
10 × 10⁵	2	19.37 ± 0.01	0.10		100.8
10 × 10⁴	1	24.15 ± 0.03	0.12	1.52	98.1	98.4
10 × 10⁴	2	23.64 ± 0.02	0.10		98.6
10 × 10³	1	28.40 ± 0.03	0.12	1.64	95.3	95.8
10 × 10³	2	27.75 ± 0.05	0.19		96.4
10 × 10²	1	33.21 ± 0.20	0.62	1.51	83.8	84.3
10 × 10²	2	32.51 ± 0.06	0.21		84.8
10 × 10¹	1	35.59 ± 0.39	1.11	0.67	102.1	99.0
	2	35.25 ± 0.06	0.17		95.7

ISSN: 1743-422X