Plasmid – standards | Day | CTMean ± SD (n=2)* | Intra-day precision | Inter-day precision | Intra-day accuracy | Inter-day accuracy |
---|---|---|---|---|---|---|
10 × 109 | 1 | 4.11 ± 0.09 | 2.22 | 0.74 | 99.9 | 100.1 |
2 | 4.17 ± 0.08 | 2.03 | 99.8 | |||
3 | 4.11 ± 0.09 | 2.36 | 99.7 | |||
4 | 4.09 ± 0.02 | 0.50 | 100.2 | |||
10 × 108 | 1 | 7.14 ± 0.07 | 1.10 | 0.92 | 102 | 102.3 |
2 | 7.24 ± 0.22 | 3.10 | 101.8 | |||
3 | 7.08 ± 0.06 | 0.88 | 102.1 | |||
4 | 7.11 ± 0.10 | 1.53 | 102.3 | |||
10 × 107 | 1 | 11.15 ± 0.05 | 0.45 | 0.85 | 101.2 | 101.4 |
2 | 11.03 ± 0.16 | 1.49 | 101.6 | |||
3 | 11.26 ± 0.12 | 1.07 | 100.8 | |||
4 | 11.19 ± 0.06 | 0.58 | 101.1 | |||
10 × 106 | 1 | 15.21 ± 0.03 | 0.20 | 0.31 | 99.8 | 100.2 |
2 | 15.10 ± 0.02 | 0.15 | 100.1 | |||
3 | 15.19 ± 0.01 | 0.11 | 100 | |||
4 | 15.19 ± 0.003 | 0.02 | 99.9 | |||
10 × 105 | 1 | 19.15 ± 0.009 | 0.04 | 0.28 | 98.5 | 98.9 |
2 | 19.03 ± 0.06 | 0.35 | 98.8 | |||
3 | 19.14 ± 0.02 | 0.10 | 98.9 | |||
4 | 19.11 ± 0.02 | 0.12 | 98.6 | |||
10 × 104 | 1 | 22.89 ± 0.009 | 0.03 | 0.30 | 97.9 | 97.8 |
2 | 22.86 ± 0.09 | 0.43 | 97.5 | |||
3 | 22.98 ± 0.13 | 0.57 | 97.9 | |||
4 | 23.00 ± 0.05 | 0.25 | 96.8 | |||
10 × 103 | 1 | 26.95 ± 0.06 | 0.24 | 0.22 | 94.2 | 94.3 |
2 | 26.87 ± 0.04 | 0.17 | 93.9 | |||
3 | 26.95 ± 0.005 | 0.01 | 95.3 | |||
4 | 27.02 ± 0.13 | 0.50 | 92.9 | |||
10 × 102 | 1 | 30.83 ± 0.16 | 0.52 | 0.35 | 89.6 | 88.2 |
2 | 30.89 ± 0.08 | 0.26 | 87.2 | |||
3 | 31.08 ± 0.03 | 0.12 | 88.5 | |||
4 | 30.97 ± 0.13 | 0.42 | 86.6 | |||
10 × 101 | 1 | 34.29 ± 0.30 | 0.89 | 0.66 | 86.3 | 92.7 |
2 | 33.78 ± 0.57 | 1.70 | 93.8 | |||
3 | 33.93 ± 0.77 | 2.29 | 97.8 | |||
4 | 33.87 ± 0.56 | 1.65 | 92.3 |