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Table 1 SYBR Green real-time PCR assay for TTV using two independent standards series

From: Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

Plasmid – standards

Day

CTMean ± SD (n=2)*

Intra-day precision

Inter-day precision

Intra-day accuracy

Inter-day accuracy

10 × 109

1

4.11 ± 0.09

2.22

0.74

99.9

100.1

2

4.17 ± 0.08

2.03

 

99.8

 

3

4.11 ± 0.09

2.36

 

99.7

 

4

4.09 ± 0.02

0.50

 

100.2

 

10 × 108

1

7.14 ± 0.07

1.10

0.92

102

102.3

2

7.24 ± 0.22

3.10

 

101.8

 

3

7.08 ± 0.06

0.88

 

102.1

 

4

7.11 ± 0.10

1.53

 

102.3

 

10 × 107

1

11.15 ± 0.05

0.45

0.85

101.2

101.4

2

11.03 ± 0.16

1.49

 

101.6

 

3

11.26 ± 0.12

1.07

 

100.8

 

4

11.19 ± 0.06

0.58

 

101.1

 

10 × 106

1

15.21 ± 0.03

0.20

0.31

99.8

100.2

2

15.10 ± 0.02

0.15

 

100.1

 

3

15.19 ± 0.01

0.11

 

100

 

4

15.19 ± 0.003

0.02

 

99.9

 

10 × 105

1

19.15 ± 0.009

0.04

0.28

98.5

98.9

2

19.03 ± 0.06

0.35

 

98.8

 

3

19.14 ± 0.02

0.10

 

98.9

 

4

19.11 ± 0.02

0.12

 

98.6

 

10 × 104

1

22.89 ± 0.009

0.03

0.30

97.9

97.8

2

22.86 ± 0.09

0.43

 

97.5

 

3

22.98 ± 0.13

0.57

 

97.9

 

4

23.00 ± 0.05

0.25

 

96.8

 

10 × 103

1

26.95 ± 0.06

0.24

0.22

94.2

94.3

2

26.87 ± 0.04

0.17

 

93.9

 

3

26.95 ± 0.005

0.01

 

95.3

 

4

27.02 ± 0.13

0.50

 

92.9

 

10 × 102

1

30.83 ± 0.16

0.52

0.35

89.6

88.2

2

30.89 ± 0.08

0.26

 

87.2

 

3

31.08 ± 0.03

0.12

 

88.5

 

4

30.97 ± 0.13

0.42

 

86.6

 

10 × 101

1

34.29 ± 0.30

0.89

0.66

86.3

92.7

2

33.78 ± 0.57

1.70

 

93.8

 

3

33.93 ± 0.77

2.29

 

97.8

 
 

4

33.87 ± 0.56

1.65

 

92.3

 
  1. *2 independent 10-fold dilution series (n=2) of plasmid standards.