Figure 5

Melting curves of an adenovirus plasmid standard using different sets of primers. The plasmid contained an insert of the human adenovirus serotype 41 hexon gene. Three sets of primers were used for amplification of the insert, producing amplicons of different lengths. Melting temperatures correlated with the lengths of amplicons. The small blue peak at around 75°C corresponds to the primer peak of the neHex3deg/neHex4deg system.