HTS of MLSMR library. High through-put screen of 313,816 compounds in single dose from the NIH MLSMR library against RSV. Compounds from the library, along with the respiratory syncytial virus, strain Long, frozen-infected HEp-2 cells were added to uninfected HEp-2 cells per well in 384-well plates. Inhibitory effects were assessed after 6 days, as described in Materials and Methods. Reference lines were calculated from the percent (%) inhibition.