Table 4 Primer sequences for detection of PCVs and TTSuVs
Primer | Primer sequence | Location (nt) | Product size (bp) |
|---|---|---|---|
PTTV1-F1 | 5’-CGGGTTCAGGAGGCTCAAT-3’ | 8–26 | 305 |
PTTV1-R1 | 5’-GCCATTCGGAACTGCACTTACT-3’ | 291–312 | |
PTTV2-F1 | 5’-TCATGACAGGGTTCACCGGA-3’ | 1–20 | 252 |
PTTV2-R1 | 5’-CGTCTGCGCACTTACTTATATACTCTA-3’ | 226–252 | |
PTTV1-F2 | CAGCGGTAGACAGAACTGTCTAGCGACT | 328–355 | < 3000 |
PTTV1-R2 | TCTGTCTACCGCTGGCGGCATAAACTCA | 314–341 | |
PTTV2-F2 | TCGAGCTCCTGAGAGCGGAGTCAAGGGGCCTA | 322–353 | < 3000 |
PTTV2-R2 | TCGAGCTCCGGCACCCGCCCAGGCGGTTAGAC | 300–331 | |
PCV1-F1 | 5’-TTGCTGAGCCTAGCGACACC-3’ | 596–615 | 350 |
PCV1-R1 | 5’-TCCACCTGCTTTCAAATCGGCC-3’ | 924–945 | |
PCV2-F1 | 5’-CAGCAAGAAGAATGGAAGAAGCGGA-3’ | 56–80 | 1057 |
PCV2-R1 | 5’-CCAGGACTACAATATCCGTGTAACT-3’ | 1088–1112 |