An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus

Table 1 Potential ligands screened for interaction with LDVICp016

Ligand(s) tested	^aKnown cellular receptor(s)	^bInteraction with
		LDVIC p016	ECTV CrmD
human / murine TNFSF1	TNFRSF1A, TNFRSF1B, TNFRSF14	no	nt
human / murine TNFSF2	TNFRSF1A, TNFRSF1B	no	yes
human / murine TNFSF3A, 3B	TNFRSF3	no	nt
human / murine TNFSF4	TNFRSF4	no	nt
human / murine TNFSF5	TNFRSF5	no	nt
human / murine TNFSF6	TNFRSF6; TNFRSF6B	no	nt
murine / murine TNFSF7	TNFRSF7	no	nt
human / murine TNFSF8	TNFRSF8	no	nt
human / murine TNFSF9	TNFRSF9	no	nt
human / murine TNFSF10	TNFRSF10A; TNFRSF10B; TNFRSF10C; TNFRSF10D; TNFRSF11B	no	nt
human / murine TNFSF11	TNFRSF11A; TNFRSF11B	no	nt
human / murine TNFSF12	TNFRSF12	no	nt
human / murine TNFSF13	TNFRSF13B; TNFRSF17	no	nt
human / murine TNFSF13B	TNFRSF13B; TNFRSF13C; TNFRSF17	no	nt
human / murine TNFSF14	TNFRSF6B; TNFRSF14, TNFRSF3	no	nt
human / murine TNFSF15	TNFRSF6B; TNFRSF25	no	nt
human APP	TNFRSF21	no	nt
Zf TNFα	zTNFR1	no	no
Fu TNFα	-	no	no
Sb TNFα	-	no	no
Cc TNFα1	-	no	yes
Cc TNFα2	-	no	no
Cc TNFα3	-	no	no
Rt RANKL	-	no	no
Rt TRAIL-like	-	no	no
Rt BALM	-	no	no

^aKnown cellular receptors listed as described by R&D Systems (http://www.rndsystems.com/Pathway.aspx?p=15485&r=15436) and[15]; ^bInteractions were tested by SPR as described by injecting a 100 nM concentration of the indicated ligands; nt: not tested. Commercial human and murine TNFSF were from R&D Systems, Inc (USA). The putative mature recombinant zebrafish (Danio rerio) TNF1 (Zf TNFα, residues 74-234, [Genbank: AY427649]) pufferfish (Takifugu rubripes) TNFα (Fu TNFα, residues 71-250 [ NCBI RefSeq: NM_001037985]), gilthead sea bream (Sparus aurata) TNFα (Sb TNFα, residues 86-253 [Genbank: AJ413189]) and common carp (Cyprinus carpio) TNFα1 (Cc TNFα1, [Genbank: CAC84641.2]), TNFα2 (Cc TNFα2, [GenBank: CAC84642.2]) and TNFα3 (Cc TNFα3, residues 62-227, [GenBank: AB112424]) were purified from baterial expression systems as described in[16]. Common carp TNFα1 (Cc TNFα1, residues 77-237 [Genbank: AJ311800]) and TNFα2 (Cc TNFα2, residues 70-231 [GenBank: AJ311801]) have been described in[17]. Rainbow trout (Rt) TNFSF have been expressed and purified as described in the text. nt: not tested.

ISSN: 1743-422X