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Figure 1 | Virology Journal

Figure 1

From: Development and validation of a quantitative real-time polymerase chain assay for universal detection of the White Spot Syndrome Virus in marine crustaceans

Figure 1

One-step PCR amplification of a 141-bp fragment of the WSSV VP28 encoding gene. (A) The ethidium bromide-stained amplification product of WSSV VP28 generated by using the VP28-140Fw and VP28-140Rv primers was electrophoresed on a 1.8% agarose gel. Lane 1: low DNA mass ladder; lane 2: 141-bp PCR amplification product of the WSSV VP28 gene. (B) Melting curve of real-time PCR for a single WSSV VP28 fragment product with a melting temperature of 82.63°C.

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