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Table 3 Summary of the method performance characteristics compared to the modified gold standard *

From: A cost effective real-time PCR for the detection of adenovirus from viral swabs

Method

Sensitivity (%; 99% CI)

Specificity (%; 99% CI)

Accuracy (%; 99% CI)

Precision (%; 99% CI)

Kappa (99% CI)

Virus culture

69.2 (56.0-69.2)

100 (96.7-100)

93.9 (88.6-93.9)

100 (80.8-100)

0.783 (0.596-0.783)

Nucleic acid extraction and real-time PCR

97.4 (86.4-97.4)

100 (97.3-100)

99.5 (95.1-99.5)

100 (88.6-100)

0.984 (0.844-0.984)

Homogenization with heat treatment and real-time PCR

100 (89.7-100)

100 (97.4-100)

100 (95.6-100)

100 (89.7-100)

1.000 (0.871-1.000)

  1. * A case was defined by concordant results (positive or negative) between at least two assays. A manual nucleic acid extraction and commercial real-time PCR was used to resolve discrepant results. TP true positive, TN true negative, FP false positive, FN false negative, CI confidence interval.
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Virology Journal

ISSN: 1743-422X