A cost effective real-time PCR for the detection of adenovirus from viral swabs

Table 1 Nucleotide sequences of primers and probes used in this study

Name	Sequence (5′ to 3′)	Reference
AdV2F	CCA GGA CGC CTC GGA GTA	[18]
AdV2R	AAA CTT GTT ATT CAG GCT GAA GTA CGT	[18]
AdV2pr	FAM- AGT TTG CCC GCG CCA CCA CCG – BHQ1^*	[18]
AdV4F	GGA CAG GAC GCT TCG GAG TA	[18]
AdV4R	CTT GTT CCC CAG ACT GAA GTA GGT	[18]
AdV4pr	FAM- CAG TTC GCC CGY GCM ACA G – BHQ1^*	[18]
FGFP	TGA TAC CCT TGT TAA TAG A	This study
RGFP	ATT GTG TGA GTTATA GTT G	This study
GFPpr1	GGT ATT GAT TTT AAA GAA GAT GG – FAM^**	This study
GFPpr2	LC705 – CAT TCT TGG GCA CAA ATT GGA- Ph^**	This study
AD1SEQ	CTG ATG TAC TAC AAC AGC ACT GGC AAC ATG GG	[32]
AD2SEQ	GCG TTG CGG TGG TGG TTA AAT GGG TTT ACG TTG TCC AT	[32]
F14MUT	TCT GCG GGT AAT TTA CTA ACT AG	This study
R14MUT	ATC TCC TGT GTT CCA GGA CCA	This study

^* The hydrolysis probes were labeled with 6-carboxyfluorescein (FAM) at the 5’end; however, the 3’end was labeled with a Black Hole Quencher 1 (BHQ1) instead of the carboxytetramethyl-rhodamine (TAMRA) quencher previously described [18].
^** Abbreviations for the hybridization probes targeting the internal control (pGFP) are as follows: [FAM], 6-carboxyfluorescein; [LC705], LightCycler-Red 705; Ph, 3′-phosphate.

ISSN: 1743-422X