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Figure 1 | Virology Journal

Figure 1

From: Intranasal immunization with a helper-dependent adenoviral vector expressing the codon-optimized fusion glycoprotein of human respiratory syncytial virus elicits protective immunity in BALB/c mice

Figure 1

Characterization of antibody responses following intranasal administration of HDAd-Fsyn in BALB/c mice. Six BALB/c mice were immunized intranasally with HDAd-Fsyn in weeks 0 and 4. ***P<0.0001. Values represent mean ± SEM. (A) Serum anti-RSV F IgG responses induced by HDAd-Fsyn. RSV F-specific antibody titers were measured by ELISA in weeks 2 and 6 after primary immunization. The results represent log10 endpoint values for six individual mice. (B) IgG subtype in sera from HDAd-Fsyn-immunized mice. Sera were obtained in week 6 after primary immunization and ELISA was performed as described in Materials and Methods. Results represent absorbance (450 nm) for samples from six individual mice. (C) Anti-RSV F IgA levels induced by HDAd-Fsyn in lung homogenates. RSV F-specific IgA antibody titers were measured by ELISA in week 6 after primary immunization. Results represent log10 endpoint values for six individual mice. (D) Virus-neutralizing activity in sera from animals vaccinated with HDAd-Fsyn. Virus-neutralizing antibody titers were analyzed by immunoenzyme assay in week 6 after primary immunization. Results are expressed as neutralizing titers corresponding to the serum dilution giving 50% inhibition of plaque formation.

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