Figure 1
From: A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
Optimization concentration of primer and probe: A: standard sample of WYMV as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM. B: standard sample of β-actin as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM. C: standard sample of 18S rRNA as template. concentration of upstream, downstream primer and probe at a)200 nM 200 nM 200 nM; b) 200 nM 200 nM 400 nM; c) 100 nM 100 nM 200 nM.