Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Interferon-α sensitizes HBx-expressing hepatocarcinoma cells to chemotherapeutic drugs through inhibition of HBx-mediated NF-κB activation

Figure 3

HBx up-regulated the expression of MDR-associated genes and anti-apoptosis genes mediated by the NF-κB pathway. A) Apoptosis index of Huh7, Huh7-3.1, and Huh7-HBx cells after treatment with ADM. The apoptosis ratio in Huh7-HBx cells was significantly lower than that of the Huh7-3.1 cells (P < 0.01). However the level of apoptosis index in the Huh7-HBx cells increased to some extent after co-treatment with the NF-κB pathway inhibitor, IMD-0354 (P<0.05). B) The expression of MDR associated genes of Huh7, Huh7-3.1 and Huh7-HBx cells was detected by real-time RT-PCR, with β-actin as internal control. The gene expression levels of Huh7 cells were set as calibrator to compare with others. C) The transcript level of anti-apoptosis genes in Huh7, Huh7-3.1, and Huh7-HBx cells was detected by real-time RT-PCR analysis with β-actin as internal control. Logarithmic scale expression values were normalized to Huh7 (ref =1, n = 3).

Back to article page

Virology Journal

ISSN: 1743-422X