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Figure 2 | Virology Journal

Figure 2

From: Interferon-α sensitizes HBx-expressing hepatocarcinoma cells to chemotherapeutic drugs through inhibition of HBx-mediated NF-κB activation

Figure 2

HBx induces NF-κB pathway activation. A) Confocal immunofluorescence analysis of Huh7-3.1, Huh7-HBx, and Huh7-HBx treated with IMD-0354 or IFN-α, using p65 antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). P65 is mainly localized in the cytoplasm of Huh7-3.1 cells, while was partly translocated into the nucleus of Huh7-HBx cells. However, the level of p65 in the nucleus of Huh7-HBx cells decreased to some extent after treatment with IMD-0354 or IFN-α. B) The expression level of phosphorylated IκBα was detected by Western blotting (lane 1, Huh7 cells; lane 2, Huh7-3.1 cells; lane 3, Huh7-HBx; lane 4, Huh7-HBx treated with IMD-0354; lane 5, Huh7-HBx treated with IFN-α).

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Virology Journal

ISSN: 1743-422X