Phylogenetic tree generated on a 125 bp overlapping stretch of the UTR of TTSuV isolates in this study and 28 reference strains. Three other Anellovirus from different species were used as outgroups in this study. The neighbor-joining (NJ) method was used to construct this tree, which was evaluated using the interior branch test (Mega 5.0). Only bootstrap support values higher than 50% are shown at each node. ML (Maximum Likelihood), MP (Maximum Parsimony), and BI (Bayesian inference) inference analysis resulted in a very similar branching pattern. GenBank accession number, source and name of isolates are shown in Additional file 1: Appendix 1. Two subtypes, PTTV1a-VA and PTTV2b-VA, are proposed subtypes for TTSuV1 and TTSuV2.