Table 5 Primers used in the construction of the PCV12 DNA clone
DNA clones | Primer | Primer Sequences (5ā-3ā)a | Primer location | Application |
|---|---|---|---|---|
| Ā | ClaI-f | CAATCGAT AACGCCTCCTTGGATACGTCATC | 1694-1724 nt | PCV1 ĪORF2 |
| Ā | AflII-r | TAACTTAAG AATAAAAACCATTACGATGTGATAAC | 995-1029 nt | Ā |
PCV12 | AflII-f | TTCCTTAAG GGTTAAGTGGGGGG | 1029-1051 nt | PCV2 ORF2 |
DNA clone | MspI-r | CGTTACCGG AGAAGAAGACAC | 1696-1716 nt | Ā |
| Ā | EcoRV-f12 | GCGATATC ATGGAGAAGAAGTTGTTGT | 649-675 nt | Duplicate |
| Ā | EcoRI-r12 | CCGAATTC TCTTTCACTTTTATAGGATG | 1721-1748 nt | Ā |