Table 2 Primers used for qRT-PCR
Target | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) |
|---|---|---|
HPV16 E7 | AGGAGGATGAAATAGATGGTCC | CTTTGTACGCACAACCGAAGC |
(nt 662–773) | ||
HPV16 E6 | AGCGACCCAGAAAGTTACCA | GCATAAATCCCGAAAAGCAA |
(nt 105–219) | ||
HPV16 E2 | GGAGACTCTTTGCCAACGTTTA | CACATTCTAGGCGCATGTGT |
(nt 2931–3080) | ||
GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG |
TBP | Hs_TBP_1_SG (Qiagen) | |
ACTB | Hs_ACTB_2_SG (Qiagen) | |