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Figure 1 | Virology Journal

Figure 1

From: Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine astrocytes

Figure 1

Expression of Coxsackievirus receptor (CAR) on primary astrocytes. Cells were treated as described in the text, fixed with Paraformaldehyde, permeabilized and stained with specific antibody. (A) A highly homogeneous population of astrocyte monolayers stained with anti-GFAP antibody (a marker for astrocytes) and Alexa Fluor 488 goat anti-rabbit secondary antibody (magnification 200×). (B) Homogeneous distribution of CAR as shown by fluorescence using anti-CAR (a marker for Coxsackievirus receptor) and Cy3-conjugated rabbit anti-mouse secondary antibody. The nuclei were counterstained with Hoechst 33258 (magnification 400×).

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