Figure 7
Competitive ELISA for determining efficiencies of phage mimotopes in blocking the HuScFv binding to rM2. In the assay, individual phage mimotope types, i.e., M2-1: ALWPPNLHAWVP, M19-1: VQIPLSYGQYYK, M23-1: HSNWDMPPIRLVAS, M23-2: QYALWPPNQAGVP, and M23-3: HSNWDMPPIRLVAS, M27-1: EDVDEIHNQSHP and M27-2: ALWPPNLHAWVP at 104, 105 and 106 pfu were mixed with individual HuScFv (5 μg) before adding to the ELISA well containing immobilized rM2 (10 μg). HuScFv mixed with irrelevant phage mimotope served as background inhibition controls. The percent ELISA inhibition was calculated. The results indicated that the mimotopes could inhibit the HuScFv binding to rM2; implying that they carried the amino acid residues analogous to the native M2 residues that could be bound by the respective HuScFv.