Table 4 Sequence details of all primer-probe combinations
Virus type | Target | Amplicon | Oligonuleotide sequence(5′-3′) | Nucleotide positions | GenBank accession no. |
|---|---|---|---|---|---|
| Â | Â | length(bp) | Forward | Â | Â |
| Â | Â | Â | Reverse | Â | Â |
| Â | Â | Â | Probe | Â | Â |
Parainfluenza 1 | HN gene | 109 | GTTGTCAATGTCTTAATTCGTATCAATAATT | 1191–1220 | U70948 |
|  |  |  | GTAGCCTACCTTCGGCACCTAA | 1278–1299 |  |
|  |  |  | TTGGAATAGTCTCGACAACAATCTTTGGCCTA | 1232–1263 |  |
Parainfluenza 2 | HN gene | 90 | GCATTTCCAATCTTCAGGACTATGA | 767–791 | D00865 |
|  |  |  | ACCTCCTGGTATAGCAGTGACTGAAC | 831–856 |  |
|  |  |  | CCATTTACCTAAGTGATGGAATCAATCGCAAA | 795–826 |  |
Parainfluenza 3 | HN gene | 136 | AGTCATGTTCTCTAGCACTCCTAAATACA | 779–807 | L25350 |
|  |  |  | ATTGAGCCATCATAATTGACAATATCAA | 887–914 |  |
|  |  |  | AACTCCCAAAGTTGATGAAAGATCAGATTATGCA | 828–861 |  |
Respiratory syncytial virus | F gene | 90 | AACAGATGTAAGCAGCTCCGTTATC | 789-813 | AF067125 |
|  |  |  | CGATTTTTATTGGATGCTGTACATTT | 853–878 |  |
| Â | Â | Â | TGCCATAGCATGACACAATGGCTCCT | 822-847 | Â |
Human metapneumovirus | N gene | 163 | CATATAAGCATGCTATATTAAAAGAGTCTC | 89-116 | AB503857 |
|  |  |  | CCTATTTCTGCAGCATATTTGTAATCAG | 224–251 |  |
| Â | Â | Â | TGCAATGATGAGGGTGTCACTGCGGTTG | 149-176 | Â |
| Â | Â | Â | TGTAATGATGAGGGTGTCACTGCGGTTG | Â | Â |
Adenoviru A | Hexon gene | 135 | GGTCTGGTGCAATTCGCC | 17818–17835 | X73487 |
|  |  |  | CACGGGCACAAAACGCA | 17936–17952 |  |
|  |  |  | CCACGGACACCTACTTCACCCTGGG | 17840–17864 |  |
Adenoviru B | Hexon gene | 138 | CGCCGGACAGGATGCTT | 45–61 | X76549 |
|  |  |  | CTACGGTCGGTGGTCAC | 166–182 |  |
|  |  |  | AGTCCGGGTCTGGTGCAGTTCGCC | 73–96 |  |
Adenoviru C | Hexon gene | 143 | ACCTGGGCCAAAACCTTCTC | 2884–2903 | J01966 |
|  |  |  | CGTCCATGGGATCCACCTC | 2940–2958 |  |
|  |  |  | AACTCCGCCCACGCGCTAGA | 2910–2929 |  |
Adenoviru D | VA gene | 75 | AAAAACGAAAGCGGTTGAGC | 2–21 | U10675 |
|  |  |  | CGGGTCGAGACGGGAGT | 128–144 |  |
|  |  |  | CCAATACCACGTTAGTCGCGGCT | 104–126 |  |
Adenoviru E | Hexon gene | 113 | CAACACCTACTCGTACAAAGTGCG | 225–248 | X84646 |
|  |  |  | TAGGTGCTGGCCATGTCCA | 281–299 |  |
|  |  |  | CGCCCACGGCCAGCGTGT | 251–268 |  |
Adenoviru F | 1.5 χ-2 gene |  | CCCGTGTTTGACAACGAAGG | 31277–31296 | L19443 |
|  |  |  | TTAGAGCTAGGCATAAATTCTACAGCA | 31363–31389 |  |
|  |  |  | ATCGACAAGGACAGTCTGCCAACACTAACG | 31326–31355 |  |