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Table 2 Sequence of primers designed for LAMP amplification of the F1L geneof ORFV

From: Comparison of a loop-mediated isothermal amplification for orf virus withquantitative real-time PCR

Primer name

Sequence (5′-3′)

Amplicon size (F2–B2c)

F3

GGTGGTCTACCCCGAGTAC

782–961a

B3

GGCGATGAACCACAGCAG

 

FIP (F1c + F2)

TAGATGGCGCCGGGCCAGA-AAACGCGGCTCAGCGG

 

BIP (B1c + B2)

CTCCTTCATGGGGCTCTTCGAC-GCAGCAGCAGCACGAG

 
  1. aNucleotide position based on the sequence of F1L generegion of ORFV isolate Jilin-Nongan (GenBank accession no.JQ271535.1).
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Virology Journal

ISSN: 1743-422X