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Table 1 Comparison of LAMP and real-time PCR for detection of orf fromintracutaneous and cohabitation infections

From: Comparison of a loop-mediated isothermal amplification for orf virus withquantitative real-time PCR

The route of infection

Samples

Days after infection

Assay

 
 

LAMP

real-time PCR

Intracutaneous

Goat 1

10

+

+

11

+

+

12

+

+

13

+

+

14

+

+

15

+

+

16

+

+

17

+

+

18

+

+

20

+

+

22

+

+

25

-

-

28

+

+

Goat 2

12

+

+

13

+

+

14

+

+

15

+

+

16

+

+

17

+

+

18

+

+

19

+

+

20

+

+

23

+

+

25

+

+

27

+

+

30

-

+

Cohabitation

Goat 3

18

+

+

21

+

+

22

+

+

23

+

+

24

+

+

25

+

+

26

+

+

27

-

-

29

+

+

Goat 4

17

+

+

19

+

+

20

+

+

21

+

+

22

+

+

23

+

+

24

+

+

25

+

+

27

+

+

29

-

-

  

32

-

-

  1. +, Positive. -, Negative.