Comparative expression of Toll-like receptors and inflammatory cytokines in pigs infected with different virulent porcine reproductive and respiratory syndrome virus isolates

Table 2 Sequences of porcine-specific real-time RT-PCR primers and TaqMan probes

Genes^a	Primer sequence (5’-3’)^b	Probe sequence (5’-3’)^c	Accession number^d
TLR2	F:TGCTGGAACCCATCGAGAA	AAGGCCATTCCCCAGCGTTTCTGTAA	GU138028
TLR2	R:AGGTAGGTCCTGGTGTTCATTATCTT	AAGGCCATTCCCCAGCGTTTCTGTAA	GU138028
TLR3	F:CCCAGTGATTCTTTTTGATACATCA	CCTGCAAAGACAGTGCCCCATTTGA	GU013759
TLR3	R:CATACTGGCACTTATCATGAAAAAGAG	CCTGCAAAGACAGTGCCCCATTTGA	GU013759
TLR7	F:CCAACAACCGGCTTGATTTAC	CAACAGCATTCGAAGAGCTACGCAACC	GU013760
TLR7	R:TCTGATTGAAAATAGTGGCTGTTACTACT	CAACAGCATTCGAAGAGCTACGCAACC	GU013760
TLR8	F: AAAAAGCACGTCCCTGAAAGAA	TTTTCAGTGGAAACCGCCTGGACCTT	GU013761
TLR8	R:TACCTGTCATCTTGGGCATTCC	TTTTCAGTGGAAACCGCCTGGACCTT	GU013761
β-actin	F:TCTTCCAGCCCTCCTTCCT	AGTCCTGCGGCATCCACGAGACC	U07786
β-actin	R:ACGTCGCACTTCATGATCGA	AGTCCTGCGGCATCCACGAGACC	U07786
18S rRNA	F:CCCCAACTTCTTAGAGGGACAA	TGGCGTTCAGCCACCCGAGATTT	DQ437859
18S rRNA	R:GGGCATCACAGACCTGTTATTG	TGGCGTTCAGCCACCCGAGATTT	DQ437859

^aTLR2, Toll-like receptor 2; TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; TLR8, Toll-like receptor 8; 18S rRNA, 18S ribosomal RNA.
^bF, forward primer; R, reverse primer.
^cAll probes contained FAM as a fluorescent reporter dye at 5’end and TAMRA as quencher dye at the 3’end.

ISSN: 1743-422X