Figure 2
From: Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells
Detection of the VP6 protein in virus infected cells. A: The VP6 protein was detected in GCRV infected CIK cells by immunostaining with mouse anti-VP6 polyclonal serum followed by Alexa Fluor® 568 donkey anti-mouse IgG (red). B: Purified GCRV particles observed by TEM. C: Western blotting analysis of the VP6 protein in virion and infected CIK cells using mouse anti-VP6 polyclonal serum. M, standard protein marker; Lane1, mock-infected CIK cell lysates; Lane 2, GCRV infected CIK cell lysates; Lane 3, purified GCRV particles; Lane 4, recombinant His-tag fusion VP6 protein; Asterisk(*) indicates VP6 (45 kDa). Arrow presents rVP6 protein (48 kDa).