Mo-MuLV infection is delayed in ZASC1−/−mice at an early time point. A.) The offspring of ZASC1+/− breeding pairs were inoculated i.p. with Mo-MuLV at p2-p3. Bone marrow from infected animals was harvested 10 days post infection along with tails to determine ZASC1 genotype. Virus infection was measured by focal immunofluorescence assay to determine infectious centers per 106 cells. Each point represents a single animal: ZASC1+/+ (circles), ZASC1+/− (squares), and ZASC1−/− (triangles). Mean average values indicated by horizontal bar. Statistically significance measured by two-tailed T-test. p-values indicated on the graph. B.) Infections were completed as in panel A, but bone marrow was pooled by genotype before magnetic cell sorting was used to purify T-cells and B-cells from the bone marrow. “Complete” is unsorted bone marrow. “T-cells” were isolated from bone marrow using α-CD90.2 conjugated Dynabeads. “B-cells” were isolated from bone marrow using α-B220 conjugated Dynabeads. Focal immunofluorescence assay was used to quantify infected cells in each population. Graph is mean average levels of infection in WT cells. N=3 litters of mice pooled by genotype. Error bars show SD. Statistical significance measured using one-way ANOVA. C.) Kaplan-Meier plot comparing survival of Mo-MuLV-infected animals of ZASC1+/+ (thick black), ZASC1+/− (thin black) and ZASC1−/− (thick gray) genotypes. Litters of mice from ZASC1+/− breeding pairs were infected p2-p3 and were allowed to develop disease. Animals that had not exhibited overt signs of disease were terminated 1 year post-infection. ZASC1+/+ (N=15), ZASC1+/− (N=31), ZASC1−/− (N=15). D.) Stacked graph of tumor types by ZASC1 genotype.