Figure 2

ZASC1 is not required for normal differentiation of splenocytes or thymocytes. Spleen and thymus from ZASC1^+/+ and ZASC1^−/− animals were harvested and stained using α-CD3, α-B220, α-CD11b, α-CD4, and α-CD8 fluorophore-conjugated antibodies and analyzed by flow cytometry. A.) Example of gating for mature cell populations after FSC/SSC analysis to gate on live cells. Top panel shows T-cell subsets (CD4+ and CD8+). Middle panel shows B-cells (B220) and T-cells (CD3). Bottom panel shows monocytes/macrophages (CD11b). B.) Graphical representation of the FACS plots shown in panel A. C.) Example of gating for CD4+, CD8+, and double positive thymocytes. D.) Graphical representation of thymocyte populations shown in panel C. N=3 mice per group with littermate controls. Error bars = SD. No statistical significance was observed between the different animal genotypes by two-tailed T-test.