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Figure 3 | Virology Journal

Figure 3

From: A simple, inexpensive, robust and sensitive dot-blot assay for equal detection of the nonstructural-1 glycoprotein of all dengue virus serotypes

Figure 3

Maximum dot-blot detection sensitivity of MAb 2C4.6 against the DENV-2 s/e NS1 glycoprotein. Serial two-fold dilutions (512 to 8 ng/ml) of immunoaffinity-purified DENV-2 s/e NS1 and YFV s/e NS1 (512 ng/ml) glycoproteins prepared in undiluted human sera were added at 10 μl volumes to paired 5-mm squares marked on a nitrocellulose membrane. After being dried and treated with 3% H2O2/H2O for 5 min to quench the endogenous human serum peroxidases, this membrane was washed and blocked. The membrane was then processed by sequential reaction steps with MAb 2C4.6, peroxidase-labelled goat anti-mouse IgG (H&L) and 4-chloro-1-naphthol/3,3’diaminobenzidine (CND) substrate. The results were obtained from one of duplicate experiments performed that gave similar results.

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Virology Journal

ISSN: 1743-422X