Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device

Table 4 Comparison of strip test with DAS ELISA for tissues collected from experimentally inoculated pigs with FMDV

Pig#/tissue	Sample dilution	Virus titer log10 TCID50	Strip test		DAS ELISA
			ESE reading	Visual	OD	Results
Serotype O
Epithelial lesion	1:4	4.05	479	++	2.208	+
(Pig14)	1:8	3.75	345	++	1.796	+
	1:16	3.45	252	++	1.207	+
	1:32	3.15	132	++	0.658	+
	1:64	2.85	60	+	0.227	+
	1:128	2.55	27	+	0.168	+
	1:256	2.25	14	-	0.06	-
Serotype A
Pig 80 Interdigital	1:2	6.7	672	++	2.405	+
	1:4	6.4	534	+	2.298	+
	1:8	6.1	407	++	2.007	+
	1:16	5.8	204	+	1.481	+
	1:32	5.5	83	+	0.884	+
	1:64	5.2	33	±	0.461	+
	1:128	4.9	33	-	0.153	+
Serotype Asia 1
Coronary	1:2	7.8	NA	++	1.989	+
Band	1:4	7.5	NA	++	1.65	+
	1:8	7.2	NA	++	1.196	+
	1:16	6.9	NA	+	0.746	+
	1:32	6.6	NA	+	0.379	+
	1:64	6.3	NA	+	0.177	+
	1:128	6.0	NA	-	0.055	-

ISSN: 1743-422X