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Table 4 Comparison of strip test with DAS ELISA for tissues collected from experimentally inoculated pigs with FMDV

From: Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device

Pig#/tissue Sample dilution Virus titer log10 TCID50 Strip test   DAS ELISA  
    ESE reading Visual OD Results
Serotype O
Epithelial lesion 1:4 4.05 479 ++ 2.208 +
(Pig14) 1:8 3.75 345 ++ 1.796 +
  1:16 3.45 252 ++ 1.207 +
  1:32 3.15 132 ++ 0.658 +
  1:64 2.85 60 + 0.227 +
  1:128 2.55 27 + 0.168 +
  1:256 2.25 14 - 0.06 -
Serotype A
Pig 80 Interdigital 1:2 6.7 672 ++ 2.405 +
  1:4 6.4 534 + 2.298 +
  1:8 6.1 407 ++ 2.007 +
  1:16 5.8 204 + 1.481 +
  1:32 5.5 83 + 0.884 +
  1:64 5.2 33 ± 0.461 +
  1:128 4.9 33 - 0.153 +
Serotype Asia 1
Coronary 1:2 7.8 NA ++ 1.989 +
Band 1:4 7.5 NA ++ 1.65 +
  1:8 7.2 NA ++ 1.196 +
  1:16 6.9 NA + 0.746 +
  1:32 6.6 NA + 0.379 +
  1:64 6.3 NA + 0.177 +
  1:128 6.0 NA - 0.055 -