Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device

Table 2 Comparison of different sizes of colloidal-gold particle conjugated detection mAb in the strip test

Gold particle size	20 nm		40 nm		60 nm
Antigen dilution	ESE reading	Visible band	ESE reading	Visible band	ESE reading	Visible band
1:16	109.93	+	244.52	++	226.84	++
1:32	63.07	+	174.40	+	237.03	++
1:64	31.57	+	95.69	+	142.10	++
1:128	16.14	-	43.23	+	88.09	+
1:256	18.79	-	19.21	-	25.43	+

FMDV Asia 1 Shamir (2-fold dilution, 50 μl) was mixed with 1 μl of each biotinylated F34Asia-8, F36Asia-14 and 6 μl mAb (F21-140SO) conjugated with different size of colloidal-gold in the running buffer. The mixture (100 μl) was applied to the gRAD. After 10 minutes, the result was recorded. + +: Strong band; +: Light band; -: No visible band.

ISSN: 1743-422X