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Table 2 Comparison of different sizes of colloidal-gold particle conjugated detection mAb in the strip test

From: Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device

Gold particle size 20 nm 40 nm 60 nm
Antigen dilution ESE reading Visible band ESE reading Visible band ESE reading Visible band
1:16 109.93 + 244.52 ++ 226.84 ++
1:32 63.07 + 174.40 + 237.03 ++
1:64 31.57 + 95.69 + 142.10 ++
1:128 16.14 - 43.23 + 88.09 +
1:256 18.79 - 19.21 - 25.43 +
  1. FMDV Asia 1 Shamir (2-fold dilution, 50 μl) was mixed with 1 μl of each biotinylated F34Asia-8, F36Asia-14 and 6 μl mAb (F21-140SO) conjugated with different size of colloidal-gold in the running buffer. The mixture (100 μl) was applied to the gRAD. After 10 minutes, the result was recorded. + +: Strong band; +: Light band; -: No visible band.