Selection of detection mAbs using a DAS ELISA. Two purified mAbs (F34Asia-8 and F36Asia-14) were coated onto microtiter plates. Then the FMDV Asia 1/Shamir and Asia 1/Pakistan 1/54 antigens were added to the plates. The antigen binding was detected with biotinylated serotype independent mAbs: (a) mAb F21-140SO and (b) mAb F14-12SA. The binding was detected with an avidin-HRP conjugate (1:2000).