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Figure 2 | Virology Journal

Figure 2

From: The 0.3-kb fragment containing the R-U5-5’leader sequence of Friend murine leukemia virus influences the level of protein expression from spliced mRNA

Figure 2

Structures of luciferase expression vectors (A). In the viral genomes, solid regions are sequences derived from the A8 virus and open regions are sequence derived from the 57 virus. The numbering of nucleotides is based on the transcript. Vectors, primers and probes used to detect the corresponding mRNA by RT-PCR are indicated on the vectors. 5’ss: 5’splice site; 3’ss: 3’splice site. Relative Luciferase activity (B) and relative amount of spliced luc-mRNA and total mRNA (C). The graphs show the mean values from 4–7 independent results and the SEM are indicated as half whiskers. The statistical comparison was carried out using the t test.

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Virology Journal

ISSN: 1743-422X