Molecular characterization of avian reovirus isolates in Tunisia

Table 1 RT-PCR primer sequences and expected PCR products

Designation	Sequence (5’-3’)	Gene	Location^a	PCR products (bp)
S1C	ATTGAATTCTCTCTGTTATCTAACCTTG	σC	446-472	738
S1D	AAGGAATTCGTTGAGAACAGAAGTAGG	σC	1183–1157
S1E	TCTGAATTCATCCGCAGCGAAGAGAGGTG	σC	630–658	342
S1F	AGTGAATTCAGTATCGCCGTGCGCAG	σB	971–943
S1G	CCTGAATTCGTGACTGACTTAACGAAC		910–936	720
S1I	ACGAATTCTTTCATTAGACATGGACC		1130–1156	520
S1H	TAAGAATTCCCAGTACGGCGCCACA		1629–1603
P1	TAACATCTAGCTATTTG		67–83	627
P2	CAAGCATTACAGGGCCAGC		720-738

ISSN: 1743-422X