Table 2 Genotyping of Pepino mosaic virus isolates with reverse transcription loop-mediated isothermal amplification (RT-LAMP) and its validation with genotype-specific RT-PCR
Country of origin | Isolate name | ELISAa | EU | US1 | CH2 |
|---|---|---|---|---|---|
RT-PCR/RT-LAMP | RT-PCR/RT-LAMP | RT-PCR/RT-LAMP | |||
USA | VFTX12-01 | (++++) | (−)/(+)b | −/− | +/+ |
VFTX12-02 | (++++) | (−)/(+)b | −/− | +/+ | |
VFTX12-03 | (++++) | (−)/(+)b | −/− | +/+ | |
VFTX12-04 | (++++) | −/− | −/− | +/+ | |
VFTX12-05 | (++++) | +/+ | −/− | +/+ | |
VFTX12-06 | (++++) | +/+ | −/− | +/+ | |
VFTX12-07 | (++++) | +/+ | −/− | +/+ | |
VFTX12-08 | (++++) | +/+ | −/− | +/+ | |
VFTX12-09 | (+++) | +/+ | −/− | +/+ | |
VFTX12-10 | (++++) | +/+ | −/− | +/+ | |
VFTX12-11 | (++++) | −/− | −/− | +/+ | |
VFTX12-12 | (++++) | −/− | −/− | +/+ | |
VFTX12-13 | (+++) | +/+ | −/− | +/+ | |
VFTX12-14 | (+++) | +/+ | −/− | +/+ | |
VFTX12-15 | (+++) | +/+ | −/− | +/+ | |
VFTX12-16 | (+++) | −/− | −/− | +/+ | |
VFTX12-18 | (+++) | −/− | −/− | +/+ | |
VFTX12-19 | (+++) | −/− | −/− | +/+ | |
VFTX12-20 | (+++) | −/− | −/− | +/+ | |
VFTX12-21 | (+) | +/+ | −/− | +/+ | |
VFTX12-22 | (+++) | −/− | −/− | +/+ | |
VFTX12-23 | (+) | −/− | −/− | +/+ | |
VFTX12-24 | (+++) | −/− | −/− | +/+ | |
VFTX12-25 | (+++) | −/− | −/− | +/+ | |
Mexico | BNMX12-01 | (+++) | −/− | +/+ | −/− |
BNMX12-02 | (+++) | −/− | +/+ | −/− | |
BNMX12-03 | (+++) | −/− | +/+ | −/− | |
BNMX12-04 | (+++) | −/− | +/+ | −/− | |
BNMX12-05 | (+++) | −/− | +/+ | −/− | |
BNMX12-06 | (+++) | −/− | +/+ | −/− | |
BNMX12-07 | (+++) | −/− | +/+ | −/− | |
BNMX12-08 | (+++) | −/− | +/+ | −/− | |
BNMX12-09 | (+++) | −/− | +/+ | −/− | |
BNMX12-10 | (+++) | −/− | +/+ | −/− | |
BNMX12-11 | (+++) | −/− | +/+ | −/− | |
BNMX12-12 | (+++) | −/− | +/+ | −/− | |
BNMX12-13 | (+++) | −/− | +/+ | +/+ | |
BNMX12-14 | (+++) | −/− | +/+ | −/− | |
BNMX12-15 | (++) | −/− | +/+ | −/− | |
BNMX12-16 | (++) | −/− | +/+ | +/+ | |
Canada | VFBC12-01 | (+++) | −/− | (−)/(+)b | +/+ |
VFBC12-02 | (++++) | −/− | (−)/(+)b | +/+ | |
VFBC12-03 | (+++) | −/− | (−)/(+)b | +/+ | |
VFBC12-04 | (−) | −/− | −/− | +/+ | |
VFBC12-05 | (+++) | −/− | −/− | +/+ | |
VFBC12-06 | (++) | −/− | −/− | +/+ | |
VFBC12-07 | (+) | −/− | −/− | +/+ | |
VFBC12-08 | (++++) | −/− | −/− | +/+ | |
VFBC12-09 | (+) | −/− | −/− | +/+ | |
VFBC12-10 | (+++) | −/− | −/− | (+)/+c | |
Positive | Â | (+++) | +/+ | +/+ | +/+ |
Negative |  | (−) | −/− | −/− | −/− |