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Figure 2 | Virology Journal

Figure 2

From: Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification

Figure 2

Phylogenetic relationship of Pepino mosaic virus isolates analyzed using the coat protein gene sequences. The GenBank accession numbers for each virus isolate used were included in each entry. The 16 PepMV isolates collected from Mexico (MX) named as BNMX12- [GenBank: JX866619 – JX866634] clustered with the US1 lineage along with the isolate recovered from a commercial rootstock seed lot designated as ‘RSSeed2’, [GenBank: JX866635]. The two additional Mexican (MX) isolates in the CH2 lineage designated as HMMX12- [GenBank: JX866636 – JX866637] were collected from a different greenhouse in Mexico. The four Canadian (BC) isolates in the CH2 genotype are designated as VFBC12- [GenBank: JX866660 – JX866663]. The U.S. (TX) isolates shown as VFTX12-, including eight isolates in the EU genotype [GenBank: JX866611 – JX866618] and 22 representative isolates in the CH2 genotype [GenBank: JX866638 – JX866659], clustered along with two CH2 sequence variants recovered from a commercial tomato seed ‘F1Seed1’ [GenBank: JX866657 in a normal CP gene and KC579401 in a 12-nt deletion mutant]. For better identification, the three sequence variants obtained from the two seed samples were labeled with asterisks.

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Virology Journal

ISSN: 1743-422X