Development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors

Table 2 Detection of RBSDV in field samples by ACP-ELISA, dot-ELISA and RT-PCR

Sample sources	RBSDV positive no./rice sample no.	RBSDV positive no./maize sample no.	RBSDV positive no./wheat sample no.	RBSDV positive no./planthopper no.
Jiangsu Province	245,245,248/250 ^a	28,28,29/30	11,11,11/12	15,15,16/123
Zhejiang Province	23,23,24/60	16,16,16/21		3,3,3/55
Shandong Province		10,10,10/15
Hainan Province	0,0,0/320			0,0,0/350
Yunnan Province	0,0,0/151	0,0,0/56		0,0,0/66
Total	268,268,272/781	54,54,55/122	11,11,11/12	18,18,19/594

^a 245, 245, 248/250 represent 245, 245, 248 of 250 samples were positive by ACP-ELISA, dot-ELISA and RT-PCR, respectively.

ISSN: 1743-422X