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Figure 3 | Virology Journal

Figure 3

From: Development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors

Figure 3

Sensitivity analyses of three MAbs determined by ACP-ELISA. A: Sensitivity analyses of three MAbs for RBSDV detection in viruliferous planthopper. Crude extracts from a RBSDV-infected small brown planthopper and a non-viruliferous planthopper (CK-) were serial two-fold diluted in 0.05 mol L-1 sodium bicarbonate buffer from1:150 to 1:38400 (individual planthopper/μL) and used as coating antigens, respectively. The OD450 value was the mean value obtained from three independent assays at 30 min after adding the substrate at room temperature. B, C, D: Sensitivity analyses of three MAbs for RBSDV detection in infected plant. RBSDV-infected plant tissue extract and healthy tissue extract (CK-) were serial two-fold diluted in 0.05 mol L-1 sodium bicarbonate buffer from 1:320 to 1:163840 (w/v, g mL-1) and used as coating antigens, respectively. The OD405 value was the mean value obtained from three independent assays at 40 min after adding the substrate at room temperature. Rice+, Wheat+ and Maize+ were RBSDV-infected rice, wheat and maize plants, respectively.

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Virology Journal

ISSN: 1743-422X