Figure 3
Activation of dendritic cells by ovalbumin-loaded particles. 5×105 bone-marrow derived DCs were co-incubated with 10 ng soluble ovalbumin, polystyrene particles loaded with 10 ng ovalbumin, or with 109 vp Ad.pIXova or UV-AdpIXova. Incubation with medium or lipopolysaccharide (LPS) was used as negative and positive controls, respectively. After 16 h co-incubation, cells were stained for expression of the activation markers CD80 and CD86 and analyzed by flow cytometry (A). Supernatants of the cells were analyzed by ELISA for interferon α and β concentrations (B). Statistically significant differences compared to untreated DCs are indicated by * (P < 0.05, Student-Newman-Keuls method).