Decreased apoptosis in p38 MAPK knockdown DENV-infected HepG2 cells. HepG2 cells were transfected either with siRNA directed against p38 MAPK or with control siRNA. Twenty-four hours post transfection, cells were infected with DENV at MOI 5 for 60 h. Cells were harvested and analyzed for (A) mRNA level of p38 MAPK (B) protein level of p38 MAPK. (C) Apoptosis by flow cytometry. Apoptosis cells have annexin V+/PI- staining. (D) Bar graph represents apoptosis experiments. All data were obtained from three independent experiments and reported as the mean ± SEM. Statistical differences between the groups were tested with an unpaired t-test using StatView version 5.0 and P value less than 0.05 was considered significant.