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Table 3 The list and sequence of primers used for PCR analysis

From: Prokaryote-expressed M2e protein improves H9N2 influenza vaccine efficacy and protection against lethal influenza a virus in mice

Primer number

Sequence

Length (base)

1

5′-CTAGCT AGCATGTCATTATTAACA-3′

24

2

5′-GAAGAT CTATGTCATTATTAACA-3′

23

3

5′-AAGCTT TAATGA GGATCC ACCTGAACCACCTGAACCACCTGAACCACCTTCAAGTTC-3

57

  1. Nucleotide bases in bold are the stop codons. Sequences of the used restriction enzyme sites are underlined.