Neutralization sensitivity of HIV-1 subtype B’ clinical isolates from former plasma donors in China

Table 1 Neutralization sensitivity of the HIV-1 B’ isolates to the plasmas from HIV-1 B’ infected patients

Virus (co-receptor usage)	Neutralizing activity (ID50 titer, 1/x) of plasma from HIV-1 B’ infected patients													VRC01^§IC50(μg/mL)	ADS-J1^#(% Inhibition)
Virus (co-receptor usage)	AH096P	AH104P	AH259P	AH311P	AH374P	AH968P	AH419P	AH628P	AH634P	AH691P	AH1188P	AH1597P	Mean	VRC01^§IC50(μg/mL)	ADS-J1^#(% Inhibition)
AH096V (R5)	11	21	204	38	57	77	21	90	―	50	―	―	49.4	>10	63.9
AH104V (X4R5)	18	―	119	14	12	―	16	20	―	98	―	―	28.1	1.107	46.9
AH259V ( R5)	―	―	―	―	―	―	―	―	―	―	―	―	―	>10	77.0
AH311V (X4R5)	22	40	≥ 648	―	18	58	214	≥ 648	166	≥ 648	―	―	207.2	1.473	92.2
AH374V (X4R5)	19	19	122	19	―	―	82	50	―	501	127	―	80.9	0.081	94.2
AH968V (R5)	26	78	167	20	39	―	50	159	21	487	20	―	90.3	0.693	86.4
AH419V (R5)	―	―	78	―	―	10	―	―	―	―	―	―	14.0	0.601	82.2
AH628V (R5)	10	10	148	―	15	112	―	―	―	38	―	―	31.8	ND	86.6
AH634V (R5)	―	―	140	―	65	120	20	―	―	263	―	―	55.3	ND	83.0
AH691V (R5)	―	―	―	―	―	10	―	―	―	―	―	―	8.2	>10	81.3
AH1188V (X4R5)	14	50	474	88	132	231	24	240	17	239	―	―	127.1	0.320	63.4
AH1597V (R5)	20	35	275	21	44	100	30	300	275	100	45	―	104.4	ND	94.7
Mean	14.3	24.4	199.3	20.7	34.5	62.5	40.8	129	45.3	204.0	22.0	―
SF33 (X4R5)*	206	156	≥ 648	≥ 648	≥ 648	80	622	441	187	399	80	≥ 648
Panel of Env-pseudotyped viruses^&
Clade B (n=8)	28.9	48.8	103.1	28.9	82.0	102.9	84.5	143.0	61.6	359.4	36.8	―	91.7
Clade C (n=4)	30.5	27.5	140.5	30.5	28.3	59.0	27.5	96.0	28.3	136.0	59.5	―	57.0
Clade A (n=4)	28.3	―	108.0	28.3	56.0	33.5	109.0	146.3	26.5	123.0	42.3	―	61.8

The plasmas were tested at a series of 3-fold dilutions ranging from 1:8 to 1:648. “―” means that the neutralizing titer is “≤ 8”. For calculation, the ID50 titers (1/x) of ≤ 8 and ≥ 648 were treated as 8 and 648, respectively. The neutralizing titers of plasmas against the autologous virus are marked in bold and italics. *A laboratory-adapted HIV-1 strain was included as a control. ^& The sensitivity of 12 plasmas were tested against Env-pseudotyped viruses of reference viruses panels[27–29], which displayed mean of ID50 titers (1/x) in the table, and “―” means that the neutralizing titer is “≤ 20” in this section (detail showed in Additional file1: Table S1). ^§VRC01 (broadly neutralizing monoclonal antibodies) was included as a control[26]. ^#ADS-J1 (HIV-1 entry inhibitor) at 10 μg/ml was included as a control of the HIV-1 inhibitor[30, 31].

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