Figure 6
From: Comparisons of the M1 genome segments and encoded μ2 proteins of different reovirus isolates
SDS-PAGE and immunoblot analyses of virion and core particles. Proteins from gradient-purified T1L (1), T2J (2), and T3D (3) particles were resolved in 5–15% SDS-polyacrylamide gels as detailed in Materials and methods. Gels were then fixed and stained with Coomassie Brilliant Blue R-250 and silver (A). Alternatively, proteins from the gels were transferred to nitrocellulose, probed with anti-μ2 antiserum (polyclonal antibodies raised against T1L μ2, kindly provided by E. G. Brown), and detected by chemiluminescence (B). Virion proteins are indicated to the left of panel A, except for μ2, which is indicated between the panels.