In vitro footprinting of RB69 gene 32-specific RNA with purified RB69 gp32 (Panels A and B) and T4 gp32 (Panels C and D). Preparation of RNA and proteins and experimental conditions for footprinting are described in METHODS. Horizontal arrows mark nucleotide positions (Fig 6B) that exhibited gp32-mediated protection from RNaseA (panels A and C) and RNase T1 (panels B and D). Darker arrows denote stronger protection. The results are summarized in Fig 6B.