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Figure 3 | Virology Journal

Figure 3

From: Murine leukemia virus (MLV) replication monitored with fluorescent proteins

Figure 3

Schematic representation of fluorescently labeled semi-replicative retroviral vectors. The env open reading frame was replaced with the gene for red fluorescent protein (RFP) in the gag/pol-expressing construct, GAG/POL-RFP, and GFP-tagged Env was expressed from a packagable vector (GFP-Env). Positions of primers used to analyze the appearance of replication-competent viruses and the stability of the inserted GFP sequences by polymerase chain reactions (PCR) are indicated as p1 to p4. SA: splice acceptor site; SD: splice donor site.

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