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Figure 4 | Virology Journal

Figure 4

From: Prevention of genital herpes in a guinea pig model using a glycoprotein D-specific single chain antibody as a microbicide

Figure 4

Western blot demonstrating expression of DL11 scFv by E, Coli. BL21 cells were transfected with p-TOPO10 containing the scFv cassette. Bacterial lysates were purified using a nickel chelation column and the reaction with anti-V5 of total lysates and various fractions from the column are shown. Lane 1, unpurified total bacterial lysate; lane 2, nickel column flow through; lanes 3 and 4, saline washes; lane 6, eluate from Ni beads; lane 7, bacterial supernatant; lane 8, scFv remaining on nickel column after elution; lane 9: supernatant from un-induced bacteria.

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Virology Journal

ISSN: 1743-422X