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  1. Research

    Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population

    The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent pr...

    Iqra Hussain, Fareeda Tasneem, Muhammed Umer, Ayesha Pervaiz, Muslim Raza, Muhammad Imran Arshad and Naveed Shahzad

    Virology Journal 2017 14:86

    Published on: 24 April 2017

  2. Research

    Epidemiology and genotype distribution of human papillomavirus (HPV) in Southwest China: a cross-sectional five years study in non-vaccinated women

    Large-size data on type-specific HPV prevalence in Southwest China are required to estimate the cervical cancer burden in the country and to prepare for HPV-based cervical screening program and further HPV vac...

    Yishu Tang, Lan Zheng, Shuangshuang Yang, Bing Li, Huiting Su and Li-ping Zhang

    Virology Journal 2017 14:84

    Published on: 21 April 2017

  3. Research

    Unique LCR variations among lineages of HPV16, 18 and 45 isolates from women with normal cervical cytology in Ghana

    In addition to being useful for classification, sequence variations of human Papillomavirus (HPV) genotypes have been implicated in differential oncogenic potential and a differential association with the diff...

    Adolf K. Awua, Richard M. K. Adanu, Edwin K. Wiredu, Edwin A. Afari, Vanessa A. Zubuch, Richard H. Asmah and Alberto Severini

    Virology Journal 2017 14:85

    Published on: 21 April 2017

  4. Research

    A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells

    Classical swine fever (CSF) is a highly contagious fatal infectious disease caused by classical swine fever virus (CSFV). A better understanding of CSFV replication is important for the study of pathogenic mec...

    Qianyi Zhang, Lu Xu, Yujie Zhang, Tuanjie Wang, Xingqi Zou, Yuanyuan Zhu, Yan Zhao, Cui Li, Kai Chen, Yongfang Sun, Junxiang Sun, Qizu Zhao and Qin Wang

    Virology Journal 2017 14:81

    Published on: 18 April 2017

  5. Research

    Tissue tropisms, infection kinetics, histologic lesions, and antibody response of the MR766 strain of Zika virus in a murine model

    The appearance of severe Zika virus (ZIKV) disease in the most recent outbreak has prompted researchers to respond through the development of tools to quickly characterize transmission and pathology. We descri...

    Anna B. Kawiecki, E. Handly Mayton, M. Fausta Dutuze, Brad A. Goupil, Ingeborg M. Langohr, Fabio Del Piero and Rebecca C. Christofferson

    Virology Journal 2017 14:82

    Published on: 18 April 2017

  6. Review

    The role of nuclear localization signal in parvovirus life cycle

    Parvoviruses are small, non-enveloped viruses with an approximately 5.0 kb, single-stranded DNA genome. Usually, the parvovirus capsid gene contains one or more nuclear localization signals (NLSs), which are r...

    Peng Liu, Shun Chen, Mingshu Wang and Anchun Cheng

    Virology Journal 2017 14:80

    Published on: 14 April 2017

  7. Research

    Study of SV40 large T antigen nucleotide specificity for DNA unwinding

    Simian Virus 40 (SV40) Large Tumor Antigen (LT) is an essential enzyme that plays a vital role in viral DNA replication in mammalian cells. As a replicative helicase and initiator, LT assembles as a double-hex...

    Damian Wang, Ana Lucia Álvarez-Cabrera and Xiaojiang S. Chen

    Virology Journal 2017 14:79

    Published on: 14 April 2017

  8. Research

    Preliminary study of the UL55 gene based on infectious Chinese virulent duck enteritis virus bacterial artificial chromosome clone

    Lethal Duck Enteritis Virus (DEV) infection can cause high morbidity and mortality of many species of waterfowl within the order Anseriformes. However, little is known about the function of viral genes includi...

    Ying Wu, Yangguang Li, Mingshu Wang, Kunfeng Sun, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Qiao Yang, Xinxin Zhao, Xiaoyue Chen and Anchun Cheng

    Virology Journal 2017 14:78

    Published on: 13 April 2017

  9. Research

    Intranasal immunization of pigs with porcine reproductive and respiratory syndrome virus-like particles plus 2′, 3′-cGAMP VacciGrade™ adjuvant exacerbates viremia after virus challenge

    Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive failure in pregnant sows and acute respiratory disease in young pigs. It is a leading infectious agent of swine respiratory compl...

    Alexandria Van Noort, April Nelsen, Angela E. Pillatzki, Diego G. Diel, Feng Li, Eric Nelson and Xiuqing Wang

    Virology Journal 2017 14:76

    Published on: 12 April 2017

  10. Research

    A guinea pig model of Zika virus infection

    Animal models are critical to understand disease and to develop countermeasures for the ongoing epidemic of Zika virus (ZIKV). Here we report that immunocompetent guinea pigs are susceptible to infection by a ...

    Mukesh Kumar, Keeton K. Krause, Francine Azouz, Eileen Nakano and Vivek R. Nerurkar

    Virology Journal 2017 14:75

    Published on: 11 April 2017

  11. Research

    Evaluation of the performance of Human Papillomavirus testing in paired urine and clinician-collected cervical samples among women aged over 30 years in Bhutan

    Urine sampling may offer a less invasive solution than cervical sampling to test for human papillomavirus (HPV) for HPV vaccine impact monitoring.

    Ugyen Tshomo, Silvia Franceschi, Tshokey Tshokey, Tashi Tobgay, Iacopo Baussano, Vanessa Tenet, Peter J. F. Snijders, Tarik Gheit, Massimo Tommasino, Alex Vorsters and Gary M. Clifford

    Virology Journal 2017 14:74

    Published on: 8 April 2017

  12. Short report

    Viral and serological kinetics in Zika virus-infected patients in South Korea

    Zika virus is a mosquito-borne flavivirus that causes clinical symptoms similar to those observed in dengue and chikungunya virus infections. The Korea Centers for Disease Control and Prevention initiated labo...

    Young Eui Jeong, Go-Woon Cha, Jung Eun Cho, Eun Ju Lee, Youngmee Jee and Won-Ja Lee

    Virology Journal 2017 14:70

    Published on: 7 April 2017

  13. Research

    Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection

    Changes in the levels of circulating microRNAs (miRNAs) in the serum of humans and animals have been detected as a result of infection with a variety of viruses. However, to date, such a miRNA profiling study ...

    Carolina Stenfeldt, Jonathan Arzt, George Smoliga, Michael LaRocco, Joseph Gutkoska and Paul Lawrence

    Virology Journal 2017 14:71

    Published on: 7 April 2017

  14. Methodology

    A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses

    Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides adva...

    Kiril M. Dimitrov, Poonam Sharma, Jeremy D. Volkening, Iryna V. Goraichuk, Abdul Wajid, Shafqat Fatima Rehmani, Asma Basharat, Ismaila Shittu, Tony M. Joannis, Patti J. Miller and Claudio L. Afonso

    Virology Journal 2017 14:72

    Published on: 7 April 2017

  15. Research

    New oligonucleotide microarray for rapid diagnosis of avian viral diseases

    We developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchit...

    Kulyaisan T. Sultankulova, Nurlan S. Kozhabergenov, Vitaliy M. Strochkov, Yerbol D. Burashev, Kamshat A. Shorayeva, Olga V. Chervyakova, Nurkuisa M. Rametov, Nurlan T. Sandybayev, Abylay R. Sansyzbay and Mukhit B. Orynbayev

    Virology Journal 2017 14:69

    Published on: 5 April 2017

  16. Research

    Antiviral efficacy of entecavir for hepatitis B virus rtA181V/T mutants

    The amino acid substitution at position 181 of the Hepatitis B virus (HBV) polymerase is a multi-drug resistance affecting both the L-nucleoside and acyclic phosphonate nucleotide groups. Data is limited on th...

    Ping Li, Jiabao Geng, Wei Li, Xiaobing Xu, Xin Zhang, Wenkai Zheng, Yuecheng Yu, Zhiguo Yang and Maorong Wang

    Virology Journal 2017 14:68

    Published on: 4 April 2017

  17. Research

    Emergence of mosaic recombinant strains potentially associated with vaccine JXA1-R and predominant circulating strains of porcine reproductive and respiratory syndrome virus in different provinces of China

    Porcine reproductive and respiratory syndrome virus (PRRSV) has caused several outbreaks in China since 2006. However, the genetic diversity of PRRSV in China has greatly increased by rapid evolution or recomb...

    Huajian Zhao, Qinggong Han, Lei Zhang, Zhiyong Zhang, Yufeng Wu, Hong Shen and Ping Jiang

    Virology Journal 2017 14:67

    Published on: 4 April 2017

  18. Methodology

    Analytical characteristics and comparative evaluation of Aptima HCV quant Dx assay with the Abbott RealTime HCV assay and Roche COBAS AmpliPrep/COBAS TaqMan HCV quantitative test v2.0

    The Aptima HCV Quant Dx assay (Aptima assay) is a fully automated quantitative assay on the Panther® system. This assay is intended for confirmation of diagnosis and monitoring of HCV RNA in plasma and serum s...

    A. Worlock, D. Blair, M. Hunsicker, T. Le-Nguyen, C. Motta, C. Nguyen, E. Papachristou, J. Pham, A. Williams, M. Vi, B. Vinluan and A. Hatzakis

    Virology Journal 2017 14:66

    Published on: 4 April 2017

  19. Research

    HPV16-E2 protein modifies self-renewal and differentiation rate in progenitor cells of human immortalized keratinocytes

    Cervical cancer is the fourth cause of death worldwide by cancer in women and is a disease associated to persistent infection with human papillomavirus (HPV), particularly from two high-risk types HPV16 and 18...

    Victoria Domínguez-Catzín, Alicia-María Reveles-Espinoza, Janet Sánchez-Ramos, Raúl Cruz-Cadena, Diana Lemus-Hernández and Efraín Garrido

    Virology Journal 2017 14:65

    Published on: 3 April 2017

  20. Review

    The reasons why Pakistan might be at high risk of Crimean Congo haemorrhagic fever epidemic; a scoping review of the literature

    Pakistan has faced a number of significant healthcare challenges over the past decade. In 2000, one of these events - a deadly epidemic of Crimean Congo Haemorrhagic Fever (CCHF) - struck Pakistan. The people ...

    Muhammad Atif, Anum Saqib, Raazeyah Ikram, Muhammad Rehan Sarwar and Shane Scahill

    Virology Journal 2017 14:63

    Published on: 23 March 2017

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