Replication kinetics of duck enteritis virus UL16 gene in vitro
© He et al.; licensee BioMed Central Ltd. 2012
Received: 19 November 2011
Accepted: 23 October 2012
Published: 21 November 2012
The function and kinetics of some herpsvirus UL16 gene have been reported. But there was no any report of duck enteritis virus (DEV) UL16 gene.
The kinetics of DEV UL16 gene was examined in DEV CHv infected duck embryo fibroblasts (DEFs) by establishment of real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) and western-blotting. In this study, UL16 mRNA was transcript at a low level from 0–18 h post-infection (p.i), and peaked at 36 h p.i. It can’t be detected in the presence of acyclovir (ACV). Besides, western-blotting analysis showed that UL16 gene expressed as an apparent 40-KDa in DEV infected cell lysate from 12 h p.i, and rose to peak level at 48 h p.i consistent with the qRT-PCR result.
These results provided the first evidence of the kinetics of DEV UL16 gene. DEV UL16 gene was a late gene and dependent on viral DNA synthesis.
Duck enteritis virus (DEV), caused duck viral enteritis (DVE), which was an acute, contagious and widespread disease in the family anatidae of the world, known as Duck plague virus (DPV), is a member of subfamily Alphaherpesvirinae of the family Herpesviridae, and has been assigned into Mardivirus genus [1, 2]. DEV is composed of four structures, linear double strand DNA, icosahedral capsid, tegument and envelope. The DEV CHv strain complete genomic sequence and gene library has been constructed in our laboratory . To date, a few of DEV genes of the kinetics has been identified and reported, such as US3 , UL31 , UL38 , UL45 , UL51 , and UTPase . There was no report of DEV UL16 gene yet. In this study, real-time quantitative reverse transcription polymerase chain reaction assay (qRT-PCR) and western-blotting were used to determine the kinetics of DEV UL16 gene.
In this work, we have firstly presented the kinetics of DEV UL16 gene. The productions of DEV UL16 gene accumulated at the late times of infection and couldn’t be detected in the presence of ACV, suggesting that the UL16 gene belonged to γ2 gene and might encode a structural protein which takes part in virion assembly, budding, and egress. The study is in accord with the earlier researches of HSV-1 UL16, HSV-2 UL16 and HCMV UL94 genes. All these results may provide some insights for further studies on the function of DEV UL16 gene.
The research was supported by grants from China 973 program(2011CB111606), Changjiang Scholars and Innovative Research Team in University (PCSIRT0848) and China Agricultural Research System (CARS-43-8).
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