Hepatitis B virus (HBV) infection is a relevant global health problem with 2 billion people that have been infected worldwide, including 350 million of them suffering from chronic HBV infection . In Latin America, the estimated HBsAg seroprevalence ranges from 0.5% to 3.0%, with the total number of HBsAg carriers approaching 11 millions . The highest prevalence surpassing 8.0 % is found among the native populations of the western Amazon basin, which includes Brazil , Colombia , Peru  and Venezuela .
HBV genome is a partially double-stranded circular DNA molecule of approximately 3,200 bp that encodes four overlapping open reading frames (ORFs) . A genetic classification based on the comparison of complete HBV genomes has identified nine genotypes, A through I , that differ by at least 8% at nucleotide level from each other. Genotype A was initially identified in southern Africa . Phylogenetic analysis of the complete genomes of subgenotype A1 isolates classified it in two clusters (African and Asian) . The introduction of subgenotype A1 into Asia could be the result of movements along the East coast of Africa, from Somalia in the horn of Africa to the Arabic Peninsula in Asia . Subgenotype A2, also denoted Ae, from “European” subgenotype, was isolated from South African carriers and is also found in Northern Europe and Greenland . Subsequently, subgenotype A3 was characterized in Cameroon, Mali and Gambia . Complete genomes of two HBV isolates from Mali (subgenotype A4) have been found to diverge by 6% from A3. Finally, four Nigerian genotype A isolates formed another group, tentatively designated subgenotype A5 . More recently, subgenotypes A6 was detected in African-Belgian patients  and subgenotype A7 was reported in Cameroon .
Genotypes B and C are predominant in East and Southeast Asia. Subgenotype D1 occurs mostly in the Mediterranean basin and Middle East. D2 has been reported in India, Japan, Europe and the United States. D3 was found in South Africa, Brazil, Rwanda, India, Costa Rica, Iran, Serbia and the United States. Finally, D4 was reported in Australia, South Africa, Somalia, Rwanda and Oceania .
Genotype E was first described in West Africa in high prevalence but in a surprisingly low diversity, as the mean diversity over the whole genome is 1.75%. HBV/E is the most prevalent genotype in western and central Africa . In Quibdó, Colombia we previously reported for the fist time the presence of this genotype in nine cases . Genotypes F and H are found in populations from Alaska to Central and South America [17, 18].
The aim of the present study was to characterize the HBV genotypes circulating in Quibdó city, Colombia. We report for the first time the HBV complete genome sequences from Afro-Colombian infected people and inferred their origin using phylogenetic analyses approaches.